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Thymineless mutagenesis in Escherichia coli.

C E Deutch, C Pauling

    Journal of Bacteriology
    |September 1, 1974
    PubMed
    Summary

    Thymine starvation in Escherichia coli causes mutations and cell death. Thymineless mutagenesis, the induction of mutations, is a more sensitive response than thymineless death, indicating they stem from the same initial event.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Thymine starvation is a condition where cells lack sufficient thymine, a crucial DNA building block.
    • This condition can lead to both cell death (thymineless death) and mutations (thymineless mutagenesis).
    • The precise relationship between these two phenomena remains incompletely understood.

    Purpose of the Study:

    • To investigate the physiological relationship between thymineless death and thymineless mutagenesis in Escherichia coli.
    • To determine the conditions under which thymine starvation induces mutations and cell death.

    Main Methods:

    • Examined the induction of arginine revertants in Escherichia coli TAU-bar under conditions of thymine starvation.
    • Assessed mutagenesis and loss of viability in various media lacking thymine, arginine, or uracil.
    • Investigated the effect of temperature (25 C and 37 C) on thymineless mutagenesis and death.

    Main Results:

    • Substantial thymineless mutagenesis occurred before the onset of thymineless death.
    • Both mutagenesis and loss of viability were inhibited by uracil supplementation.
    • Mutagenesis occurred even at lower temperatures with minimal cell death, and at suboptimal thymine concentrations at 37 C.
    • Mutagenesis was not localized to the DNA replication fork.

    Conclusions:

    • Thymineless mutagenesis and thymineless death are initiated by the same primary event.
    • Mutagenesis is a more sensitive indicator of this event than cell death.
    • The mechanism of thymineless mutagenesis likely involves more than simple base-pairing errors due to thymine absence.

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