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Quantitative large-volume sampling technique.

M A Levin, J R Fischer, V J Cabelli

    Applied Microbiology
    |September 1, 1974
    PubMed
    Summary
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    A new filtration method allows for high-volume water sampling to accurately count microorganisms, even at very low levels. This technique enhances the most probable number procedure for precise microbial quantitation.

    Area of Science:

    • Environmental science
    • Microbiology
    • Analytical chemistry

    Background:

    • Accurate microbial water quality monitoring is crucial for public health.
    • Detecting microorganisms at low densities presents significant challenges for traditional methods.

    Purpose of the Study:

    • To develop and validate a novel filtration technique for high-volume water sampling.
    • To improve the quantitation of microorganisms present at very low densities in water samples.

    Main Methods:

    • A high-volume filtration system was designed for water sample processing.
    • The filtration technique was integrated with the most probable number (MPN) procedure.
    • Microbial analysis was performed on water samples with varying low microbial concentrations.

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    Main Results:

    • The developed filtration technique effectively processed large volumes of water.
    • Integration with the MPN procedure enabled accurate quantitation of low-density microbial populations.
    • The method demonstrated high sensitivity for detecting scarce microorganisms.

    Conclusions:

    • The novel filtration technique, combined with the MPN procedure, offers a robust solution for quantifying low microbial loads in water.
    • This approach significantly advances the ability to monitor microbial contamination in large water volumes.
    • The method is suitable for environmental monitoring and public health applications requiring sensitive microbial detection.