Action of dimeric hybrids of native and selectively alkylated ribonuclease A on double-stranded ribonucleic acid

The hypothesis that both active sites of dimeric ribonuclease A have to be simultaneously available in order to make the enzyme able to degrade double-stranded RNA is tested with purified hybrid dimers of ribonuclease A and its carboxymethylated derivative. The results obtained support the hypothesis.