Autoradiographic and fluorescent staining studies of bone marrow chromosomes from a patient with acute granulocytic...

Results of cytogenetic studies of a patient with acute granulocytic leukemia are described. The modal number of chromosomes in cells from bone marrow samples was 43; the karyotype was characterized by missing chromosomes in groups B, C, and E. According to successive studies of the same metaphases by fluorescent staining and autoradiographic techniques, the labeling pattern of chromosomes in leukemic cells was generally similar to those observed in normal cultured leukocytes, whereas the fluorescent pattern of leukemic chromosomes was rather indistinct, and the bright fluorescent pattern of the Y chromosome was not apparent.