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Characterization of a plasmid determining resistance to erythromycin, lincomycin, and vernamycin Balpha in a strain
Antimicrobial Agents and Chemotherapy
|May 1, 1974
Abstract:
A plasmid determining resistance to erythromycin, lincomycin, and vernamycin B(alpha) was isolated from a strain of Streptococcus pyogenes. The plasmid has a molecular weight of approximately 17 x 10(6) and is present to the extent of one to two copies per chromosomal genome equivalent.
Insights
A novel plasmid conferring resistance to multiple antibiotics, including erythromycin and lincomycin, was identified in Streptococcus pyogenes. This discovery aids in understanding antibiotic resistance mechanisms in bacteria.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Antibiotic resistance is a growing global health concern.
- Streptococcus pyogenes is a significant human pathogen.
- Plasmids are key vectors for the spread of antibiotic resistance.
Purpose of the Study:
- To isolate and characterize a plasmid responsible for antibiotic resistance in Streptococcus pyogenes.
- To determine the molecular weight and copy number of the identified plasmid.
Main Methods:
- Isolation of a resistance-conferring plasmid from Streptococcus pyogenes.
- Molecular weight determination of the plasmid.
- Estimation of plasmid copy number relative to the chromosome.
Main Results:
- A plasmid conferring resistance to erythromycin, lincomycin, and vernamycin B(alpha) was successfully isolated.
- The plasmid has an estimated molecular weight of 17 x 10^6 Daltons.
- The plasmid exists at one to two copies per chromosomal genome equivalent.
Conclusions:
- Streptococcus pyogenes can harbor plasmids that confer resistance to clinically relevant antibiotics.
- The characterized plasmid represents a potential mechanism for the dissemination of antibiotic resistance within bacterial populations.