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Related Experiment Video

Updated: Jun 1, 2026

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

Platelet interaction with polymerizing fibrin.

S Niewiarowski, E Regoeczi, G J Stewart

The Journal of Clinical Investigation

|March 1, 1972

Summary

Platelets interact with fibrinogen intermediates during its conversion to fibrin, influencing clot formation. This interaction, crucial for hemostasis, involves platelets adhering to polymerizing fibrin fibers.

Area of Science:

Hematology
Biochemistry
Cell Biology

Background:

Platelets play a critical role in hemostasis and thrombosis.
Fibrinogen undergoes polymerization to form fibrin, the main structural component of blood clots.
The interaction between platelets and fibrinogen/fibrin is complex and not fully understood.

Purpose of the Study:

To investigate the interaction between platelets and fibrinogen during its transition to fibrin.
To elucidate the mechanisms and consequences of platelet association with polymerizing fibrin.
To determine the effect of platelets on fibrin polymerization.

Main Methods:

Photometric, isotopic, and electron microscopic techniques were employed.
Washed platelets from pigs, rabbits, and humans were used.

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Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

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Last Updated: Jun 1, 2026

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

Published on: April 2, 2013

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

Live-cell Imaging of Platelet Degranulation and Secretion Under Flow

Published on: July 10, 2017

Fibrinogen was incubated with enzymes like reptilase and thrombin to induce polymerization.

Main Results:

Platelets readily associated with intermediate fibrinogen products, leading to aggregation.
Platelet interaction with polymerizing fibrin caused minor release of platelet constituents, not via the release reaction.
Platelets influenced fibrin fiber formation, suggesting an effect on fibrin polymerization.

Conclusions:

Platelets interact with fibrinogen intermediates and polymerizing fibrin, contributing to clot structure.
This interaction is distinct from the platelet release reaction and ADP-induced aggregation.
Platelet adherence to fibrin may be essential for forming links in hemostatic plugs and thrombi.