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Related Experiment Videos

Antigenic relationship between the fourth component of human and guinea pig complement.

S H Ohanian, T Borsos

    Journal of Immunology (Baltimore, Md. : 1950)
    |January 1, 1975
    PubMed
    Summary

    Cell-bound human and guinea pig complement component 4 (C4) show antigenic similarities. This cross-reactivity may arise from conformational changes due to cell surface binding, unlike soluble C4.

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    Area of Science:

    • Immunology
    • Complement System Biology

    Background:

    • The complement system is a crucial part of innate immunity.
    • Complement component 4 (C4) plays a key role in complement activation.
    • Understanding C4 interactions is vital for immune response studies.

    Purpose of the Study:

    • To investigate antigenic similarities between human and guinea pig cell-bound C4.
    • To determine if cell surface binding alters C4 antigenicity.

    Main Methods:

    • Utilized antisera specific to human and guinea pig C4.
    • Tested C4 from normal sera and after C1 complex treatment.
    • Examined cell-bound C4 for antigenic cross-reactivity.

    Main Results:

    • Detected antigenic similarity between cell-bound human and guinea pig C4.

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  • Observed no cross-reactivity between soluble C4 from either species.
  • No cross-reactivity was found after treatment with antigen-antibody C1 complexes.

    • Conclusions:

    • Cell surface binding induces conformational changes in C4, leading to detectable antigenic similarities between species.
    • Soluble C4 molecules retain species-specific antigenic determinants.
    • This finding highlights the impact of cellular microenvironments on complement protein structure and function.