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Updated: Aug 18, 2026

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
Published on: November 16, 2016
An osmotically fragile mutant of Bacillus subtilis with an active membrane-associated phospholipase A 1
Abstract:
By use of a newly developed procedure for the isolation of mutants with osmotically fragile protoplasts, a mutant of Bacillus subtilis was isolated that has a very active enzyme system for the catabolism of phospholipids via the sequential action of a phospholipase A(1) (EC 3.1.1.4) and a lysophospholipase (EC 3.1.1.5). The wild-type bacteria contain no detectable phospholipase A(1) activity, but do contain a protein that specifically inhibits the phospholipase A(1) in the mutant. This protein may play an important role in the control of phospholipid catabolism.
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