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Hyperprotease-producing mutants of Bacillus subtilis.

T B Higerd, J A Hoch, J Spizizen

    Journal of Bacteriology
    |November 1, 1972
    PubMed
    Summary
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    Proceedings of the National Academy of Sciences of the United States of America·2001

    Researchers developed Bacillus subtilis mutants with significantly enhanced extracellular protease production, showing a 16- to 37-fold increase in proteolytic activity. These mutants exhibit elevated neutral and alkaline protease levels, genetically linked to the argC4 marker.

    Area of Science:

    • Microbiology
    • Biotechnology
    • Enzymology

    Background:

    • Bacillus subtilis is a key industrial microorganism.
    • Extracellular proteases are valuable enzymes with diverse applications.
    • Enhancing protease production in B. subtilis is of significant biotechnological interest.

    Purpose of the Study:

    • To isolate and characterize Bacillus subtilis mutants with increased extracellular protease production.
    • To quantify the level of protease activity enhancement in the isolated mutants.
    • To investigate the genetic basis of the enhanced protease production.

    Main Methods:

    • Isolation of Bacillus subtilis mutants.
    • Cultivation of mutants and wild-type strains.
    • Analysis of culture supernatants for proteolytic activity.

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  • PBS-1 transduction analysis for genetic mapping.

    • Main Results:

    • Several Bacillus subtilis mutants with elevated extracellular protease production were successfully isolated.
    • Proteolytic activity in mutant supernatants increased 16- to 37-fold compared to the wild strain.
    • The enhanced activity resulted from simultaneous increases in both neutral and alkaline protease levels.
    • Genetic analysis indicated that all characterized mutants were linked to the argC4 marker.

    Conclusions:

    • Mutants of Bacillus subtilis capable of hyper-production of extracellular proteases have been developed.
    • The genetic linkage to the argC4 marker provides a basis for further genetic studies and strain improvement.
    • These hyper-producing mutants hold potential for industrial applications requiring high levels of protease enzymes.