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Mutation in Shigella flexneri 2a resulting in increased electrophoretic mobility.

L M Corwin, L A Talevi

    Infection and Immunity
    |May 1, 1972
    PubMed
    Summary

    Calcium ions increase the negative charge of an avirulent Shigella flexneri mutant, enhancing its stability. This altered bacterial surface charge impacts electrophoretic mobility and lipopolysaccharide protection.

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    Area of Science:

    • Microbiology
    • Bacterial genetics
    • Surface chemistry

    Background:

    • Shigella flexneri 2a is a pathogen causing bacillary dysentery.
    • Bacterial surface properties, such as charge, influence virulence and host interactions.
    • Calcium ions are essential for bacterial growth and can affect cell surface characteristics.

    Purpose of the Study:

    • To investigate the effect of calcium ions on the surface charge of an avirulent Shigella flexneri 2a mutant.
    • To compare the electrophoretic mobility of the avirulent mutant with its virulent parent strain under different growth conditions.
    • To determine the role of surface charge alterations in the stability of the avirulent strain.

    Main Methods:

    • Culturing Shigella flexneri 2a strains (virulent and avirulent mutant) in broth with and without calcium ions.
    • Measuring electrophoretic mobility using an electrophoretic apparatus.
    • Assessing the effect of ethylenediaminetetraacetic acid (EDTA) on electrophoretic mobility and lipopolysaccharide stability.

    Main Results:

    • The avirulent mutant exhibited a 3- to 4-fold increase in negative charge (electrophoretic mobility) compared to the virulent parent when grown in the presence of calcium ions.
    • Ethylenediaminetetraacetic acid (EDTA) increased the electronegativity of the avirulent strain but had no effect on the virulent strain.
    • In the absence of calcium, no significant differences in electrophoretic mobility were observed between the strains.
    • The increased negative charge stabilized the avirulent strain against lipopolysaccharide loss induced by EDTA.

    Conclusions:

    • Calcium ions induce significant alterations in the surface charge of an avirulent Shigella flexneri 2a mutant.
    • The enhanced negative charge is associated with increased stability against EDTA-induced lipopolysaccharide loss.
    • These findings suggest a potential role for surface charge modifications in bacterial avirulence and stability.

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