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Related Experiment Videos

Microtitration of Bacillus cereus hemolysin.

J C Coolbaugh, R D Wende, R P Williams

    Applied Microbiology
    |December 1, 1972
    PubMed
    Summary

    A new microtiter method accurately quantifies Bacillus cereus hemolysin. This assay is crucial for understanding bacterial toxins and food safety.

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    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Bacillus cereus produces hemolysin, a toxin that affects red blood cells.
    • Accurate quantification of hemolysin is important for food safety and clinical diagnostics.

    Purpose of the Study:

    • To describe a novel microtiter procedure for quantifying Bacillus cereus hemolysin.
    • To provide a reliable method for researchers studying bacterial toxins.

    Main Methods:

    • A microtiter plate-based assay was developed.
    • Hemolytic activity was measured spectrophotometrically.

    Main Results:

    • The procedure allows for sensitive and reproducible quantification of Bacillus cereus hemolysin.
    • The method is adaptable for high-throughput screening.

    Conclusions:

    • The described microtiter procedure offers an efficient and accurate means to quantify Bacillus cereus hemolysin.
    • This method can aid in the detection and characterization of Bacillus cereus toxins.

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