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Host range and properties of the Pseudomonas aeruginosa R factor R1822
Journal of Bacteriology
|February 1, 1973
Abstract:
R1822, a plasmid specifying multiple drug resistances, has been transferred to a variety of species representative of related and unrelated genera. The host range of the plasmid includes Enterobacteriaceae, soil saprophytes, Neisseria perflava, and photosynthetic bacteria. With the acquisition of drug resistance(s), these strains became sensitive to a small, ribonuclease-sensitive bacteriophage, designated PRR1, isolated by enrichment from sewage.
Insights
The R1822 plasmid, conferring multiple drug resistances, successfully transferred to diverse bacterial genera. Acquired resistance made these bacteria susceptible to the bacteriophage PRR1.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Plasmids are crucial for horizontal gene transfer in bacteria.
- Multiple drug resistance (MDR) plasmids pose significant public health challenges.
- Understanding plasmid host range is key to controlling their spread.
Purpose of the Study:
- To investigate the host range of the R1822 multiple drug resistance plasmid.
- To determine the impact of R1822 acquisition on bacterial susceptibility to bacteriophages.
Main Methods:
- Conjugal transfer of the R1822 plasmid to various bacterial species.
- Testing bacterial susceptibility to bacteriophage PRR1.
- Characterization of bacteriophage PRR1 as small and ribonuclease-sensitive.
Main Results:
- The R1822 plasmid was successfully transferred to diverse genera, including Enterobacteriaceae, soil saprophytes, Neisseria perflava, and photosynthetic bacteria.
- Acquisition of R1822 conferred multiple drug resistances.
- Transconjugant strains exhibited increased sensitivity to bacteriophage PRR1.
Conclusions:
- The R1822 plasmid has a broad host range across different bacterial groups.
- Acquisition of MDR plasmids can alter bacterial susceptibility to phage predation.
- Bacteriophage PRR1 shows potential for targeting bacteria carrying the R1822 plasmid.