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Prophage-mediated interference affecting the development of Bacillus subtilis bacteriophage phi e.

C W Rettenmier, H E Hemphill

    Journal of Virology
    |March 1, 1973
    PubMed
    Summary

    Bacteriophage phie plating efficiency decreases on Bacillus subtilis lysogenic for bacteriophage SP02. This suggests a novel restriction mechanism, distinct from typical host-controlled modification, prompting the isolation of high-efficiency mutants.

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    Area of Science:

    • Microbiology
    • Virology
    • Bacteriophage research

    Background:

    • Bacteriophages are viruses that infect bacteria.
    • Lysogenic bacteria harbor bacteriophage DNA integrated into their genome.
    • Bacteriophage SP02 is a temperate bacteriophage known to lysogenize Bacillus subtilis.

    Purpose of the Study:

    • To investigate the reduced plating efficiency of bacteriophage phie on Bacillus subtilis lysogenic for bacteriophage SP02.
    • To determine if the observed phenomenon is due to prophage-mediated restriction or host-controlled modification.
    • To isolate mutants of bacteriophage phie that overcome this restriction.

    Main Methods:

    • Efficiency of plating assays were performed.
    • Lysogenic Bacillus subtilis strains were used as hosts.

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  • Mutagenesis and selection were employed to isolate mutant phages.

    • Main Results:

    • Bacteriophage phie exhibited significantly reduced plating efficiency on Bacillus subtilis strains lysogenic for SP02.
    • Host-controlled modification of phie was not detected, differentiating the phenomenon from known restriction systems.
    • Mutants of phie were successfully isolated, demonstrating high plating efficiency on the lysogenic host.

    Conclusions:

    • The reduced plating efficiency of phie on SP02-lysogenic Bacillus subtilis is not due to standard host-controlled modification.
    • A novel form of prophage-mediated restriction may be involved.
    • The isolated phie mutants provide tools to further study this unique phage-host interaction.