Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid methods for extracting autolysins from Bacillus subtilis.

W C Brown

    Applied Microbiology
    |February 1, 1973
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A nano particle vector comprised of poly lactic-co-glycolic acid and monophosphoryl lipid A and recombinant Mycobacterium avium subsp paratuberculosis peptides stimulate a pro-immune profile in bovine macrophages.

    Journal of applied microbiology·2017
    Same author

    Search for patterns by combining cosmic-ray energy and arrival directions at the Pierre Auger Observatory.

    The European physical journal. C, Particles and fields·2015
    Same author

    Measurement of the proton-air cross section at √s=57 TeV with the Pierre Auger Observatory.

    Physical review letters·2012
    Same author

    Point-spread function associated with underwater imaging through a wavy air-water interface: theory and laboratory tank experiment.

    Applied optics·2010
    Same author

    Osteotomy in a Man of Forty-Two.

    British medical journal·2010
    Same author

    Wandering OEdema.

    British medical journal·2010

    We present two effective methods for extracting bacterial autolysins from whole cells using lithium chloride or Triton X-100. These techniques enable sensitive detection of autolytic activity and large-scale enzyme isolation.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Enzymology

    Background:

    • Autolysins are crucial enzymes involved in bacterial cell wall metabolism.
    • Efficient extraction methods are needed for studying autolysin activity and for large-scale purification.

    Purpose of the Study:

    • To describe and compare two novel procedures for extracting autolysins from whole bacterial cells.
    • To evaluate the efficiency of these methods for various bacterial species and for detecting subtle differences in enzyme activity.

    Main Methods:

    • Extraction of autolysins from whole bacterial cells using 5 M lithium chloride (LiCl) at 4°C.
    • Extraction of autolysins using 2% Triton X-100 detergent.
    • Comparison of enzyme yields and characterization of autolysin properties, including pH optima.

    Related Experiment Videos

    Main Results:

    • The LiCl method yielded significantly more autolysin compared to cell wall autolysis or salt extraction of cell walls.
    • The Triton X-100 method was less efficient than LiCl but comparable to cell wall autolysis.
    • Both methods successfully extracted autolysins from multiple bacterial species and revealed enzymes with multiple pH optima.

    Conclusions:

    • Lithium chloride and Triton X-100 are effective for bacterial autolysin extraction from whole cells.
    • These methods offer sensitivity for detecting differences in autolytic activity and are suitable for large-scale autolysin isolation and characterization.