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Identification of polysomal RNA in BHK cells infected by sindbis virus
Journal of Virology
|April 1, 1973
Abstract:
Polysomes were prepared from Sindbis virus-infected BHK cells. The major species of RNA in these polysomes was identified as 26S RNA (interjacent RNA) by (i) disrupting the polysomes with EDTA; (ii) treating the infected cells with puromycin; and (iii) isolating polysomes from cells infected with a temperature-sensitive mutant that does not form nucleocapsids. Small amounts of 42S RNA and 33S RNA were also found in polysomes.
Insights
Researchers identified 26S RNA as the primary component in polysomes from Sindbis virus-infected cells. This finding clarifies the RNA species involved in viral protein synthesis in BHK cells.
Area of Science:
- Virology
- Molecular Biology
- Cell Biology
Background:
- Sindbis virus is an RNA virus that infects various cell types.
- Polysomes are complexes of messenger RNA and ribosomes involved in protein synthesis.
Purpose of the Study:
- To identify the major RNA species present in polysomes of Sindbis virus-infected cells.
- To understand the role of different RNA species in viral replication.
Main Methods:
- Preparation of polysomes from Sindbis virus-infected Baby Hamster Kidney (BHK) cells.
- Disruption of polysomes using Ethylenediaminetetraacetic acid (EDTA).
- Treatment of infected cells with puromycin.
- Isolation of polysomes from cells infected with a temperature-sensitive Sindbis virus mutant.
Main Results:
- The predominant RNA species found in polysomes was 26S RNA, also known as interjacent RNA.
- Minor amounts of 42S RNA and 33S RNA were also detected in the polysomes.
- Experimental conditions confirmed the identity of 26S RNA as the major polysomal RNA.
Conclusions:
- 26S RNA is the primary messenger RNA responsible for Sindbis virus protein synthesis in BHK cells.
- The study provides insights into the composition of viral RNA complexes during infection.