Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conformational changes in human lens proteins in cataract.

J J Harding

    The Biochemical Journal
    |August 1, 1972
    PubMed
    Summary

    Cataract formation involves changes in human lens proteins, indicated by increased thiol group reactivity and tryptic digestion susceptibility. This suggests protein unfolding occurs during cataractogenesis, altering protein structure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Characterization and management of ERK inhibitor associated dermatologic adverse events: analysis from a nonrandomized trial of ulixertinib for advanced cancers.

    Investigational new drugs·2021
    Same author

    Characterization of on-target adverse events caused by TRK inhibitor therapy.

    Annals of oncology : official journal of the European Society for Medical Oncology·2020
    Same author

    Phase III randomized study of second line ADI-PEG 20 plus best supportive care versus placebo plus best supportive care in patients with advanced hepatocellular carcinoma.

    Annals of oncology : official journal of the European Society for Medical Oncology·2018
    Same author

    Comments on the role of metal-catalysed autoxidation in glycation and the Maillard reaction.

    Redox report : communications in free radical research·2016
    Same author

    Investigation of gammaE-crystallin target protein binding to bovine lens alpha-crystallin by small-angle neutron scattering.

    Biochimica et biophysica acta·2009
    Same author

    Effect of carnosine, aminoguanidine, and aspirin drops on the prevention of cataracts in diabetic rats.

    Molecular vision·2008

    Area of Science:

    • Biochemistry
    • Ophthalmology
    • Proteomics

    Background:

    • Human lens proteins undergo structural changes during cataractogenesis.
    • Understanding these changes is crucial for developing targeted therapies.

    Purpose of the Study:

    • To investigate the reactivity of protein thiol groups in human lens proteins.
    • To assess the susceptibility of lens proteins to tryptic digestion in normal and cataractous lenses.
    • To determine the accessibility of tyrosine residues in normal human lens proteins.

    Main Methods:

    • Assessing protein thiol group reactivity.
    • Evaluating susceptibility to tryptic digestion.
    • Utilizing tetranitromethane reaction to probe tyrosine accessibility.

    Main Results:

    • Increased reactivity of protein thiol groups was observed in some cataractous lenses.
    • Enhanced susceptibility to tryptic digestion was noted in proteins from cataractous lenses.
    • Nearly all tyrosine residues in normal human lens proteins reacted with tetranitromethane, suggesting surface exposure.

    Conclusions:

    • Protein unfolding is a likely event during cataractogenesis.
    • Altered protein structure in cataracts may affect lens transparency.
    • Tyrosine residues are predominantly located on the exterior of major normal human lens proteins.

    Related Experiment Videos