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A simple polychrome stain for conventionally fixed Epon-embedded tissues.

J Van Reempts, M Borgers

Stain Technology

|January 1, 1975

Summary

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This study presents a one-step Mallory-Heidenhain staining technique for routine use on various Epon embedded tissues. The method effectively differentiates cellular and extracellular components, including nuclei, cytoplasm, collagen, and elastic fibers.

Area of Science:

Histology
Biochemistry
Cell Biology

Background:

Routine histological staining is crucial for visualizing tissue architecture.
Existing methods may lack specificity or require multiple steps for complex tissue analysis.
The Mallory-Heidenhain stain is a well-established technique for demonstrating connective tissue elements.

Purpose of the Study:

To develop and validate a simplified, one-step Mallory-Heidenhain staining protocol.
To enhance the routine visualization of diverse tissue and cellular components in electron microscopy-prepared samples.
To provide a reliable staining method for glutaraldehyde or OsO4 fixed, Epon embedded tissues.

Main Methods:

A one-step Mallory-Heidenhain staining procedure was adapted.

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Sections underwent a brief hydrogen peroxide (H2O2) treatment.

Nuclear staining was performed with celestine blue B.

A final staining step utilized a modified Cason's solution.

Main Results:

Nuclei were stained dark brown.
Cytoplasm appeared yellow.
Collagen fibers were distinctly red.
Elastic fibers were visualized in blue.
Intracytoplasmic glycogen stained blue, mucus stained violet.
Leukocyte granules were orange to red.

Conclusions:

The described one-step Mallory-Heidenhain stain is a robust and efficient method for routine tissue analysis.
This technique offers excellent differentiation of various tissue and cellular structures.
It is suitable for a wide range of organs and fixation methods commonly used in histology.