Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

C3, GBG, orosomucoid and haptoglobin polymorphisms. Improved staining methods.

N Tulchin, L Ornstein, P Weiner

    Vox Sanguinis
    |January 1, 1975
    PubMed
    Summary

    This study presents improved electrophoretic methods for analyzing genetic polymorphisms in complement C3, properdin factor B (GBG), and orosomucoid. Enhanced staining and a novel haptoglobin typing technique increase efficiency and resolution.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    C-myc protein distribution - mammary adenocarcinomas of mtv/myc transgenic mice.

    International journal of oncology·2011
    Same author

    Immunohistologic c-myc protein in benign breast disease and cancer.

    International journal of oncology·2011
    Same author

    [Acute malaria in an Israeli tourist to Kenya].

    Harefuah·2004
    Same author

    Inspiratory muscle training as a tool for the management of patients with COPD.

    The European respiratory journal·2004
    Same author

    The relation between preprocedural C-reactive protein levels and early and late complications in patients with acute myocardial infarction undergoing interventional coronary angioplasty.

    Clinical cardiology·2004
    Same author

    Maintenance of inspiratory muscle training in COPD patients: one year follow-up.

    The European respiratory journal·2004

    Area of Science:

    • Biochemistry
    • Genetics
    • Molecular Biology

    Background:

    • Accurate typing of genetic polymorphisms is crucial for various biological and clinical studies.
    • Traditional electrophoretic methods can be limited by sensitivity and resolution.
    • Developing efficient and high-resolution techniques is essential for genetic analysis.

    Purpose of the Study:

    • To optimize electrophoretic methods for analyzing C3, GBG, and orosomucoid polymorphisms.
    • To enhance protein staining sensitivity and resolution in electrophoresis.
    • To develop a novel method for haptoglobin typing.

    Main Methods:

    • High-voltage agarose gel electrophoresis was employed for typing C3, GBG, and orosomucoid.
    • Coomassie blue dye was utilized for protein staining, offering increased sensitivity and resolution compared to amido black.

    Related Experiment Videos

  • A new method for haptoglobin typing was developed, leveraging the peroxidase activity of the haptoglobin-hemoglobin complex.

    • Main Results:

    • The high-voltage agarose method allowed for the typing of 15-20 samples efficiently.
    • Coomassie blue staining provided higher resolution results than amido black.
    • The developed haptoglobin typing method proved effective.

    Conclusions:

    • The optimized electrophoretic techniques enhance the efficiency and accuracy of genetic polymorphism analysis.
    • The improved staining and novel haptoglobin typing method offer significant advantages for genetic research.
    • These advancements contribute to more precise genetic characterization and potential clinical applications.