Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Flow-injection analysis with chemiluminescence detection.

G Rule, W R Seitz

    Clinical Chemistry
    |September 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Fluorescence polarization studies of perylene/fulvic acid binding.

    Environmental science & technology·2012
    Same author

    Effects of structural and compositional variations of dissolved humic materials on pyrene Koc values.

    Environmental science & technology·2011
    Same author

    A magnetostatic-coupling based remote query sensor for environmental monitoring.

    Journal of physics D: Applied physics·2002
    Same author

    A remotely interrogatable sensor for chemical monitoring.

    IEEE transactions on magnetics·2001
    Same author

    A 384-well solid-phase extraction for LC/MS/MS determination of methotrexate and its 7-hydroxy metabolite in human urine and plasma.

    Analytical chemistry·2001
    Same author

    High-throughput sample preparation and analysis using 96-well membrane solid-phase extraction and liquid chromatography-tandem mass spectrometry for the determination of steroids in human urine.

    Journal of the American Society for Mass Spectrometry·1999
    Same journal

    Comparison of Information-Dependent Acquisition and Sequential Window Acquisition of All Theoretical Mass Spectra for Untargeted Drug Testing on a Linear Ion Trap-Pulsing Quadrupole-Time of Flight Mass Spectrometer.

    Clinical chemistry·2026
    Same journal

    Patterns of One-Year Change in HbA1c and Continuous Glucose Monitoring (CGM) Metrics in Older Adults with Type 2 Diabetes.

    Clinical chemistry·2026
    Same journal

    TSH Pediatric Reference Intervals: Lack of CALIPER Applicability to US-Based Populations.

    Clinical chemistry·2026
    Same journal

    Rapid Detection of Hemoglobinopathy Variants Using One-Step Library Preparation and Nanopore Sequencing.

    Clinical chemistry·2026
    Same journal

    Editor's Note: Circulating Proteolytic Products of Carboxypeptidase N for Early Detection of Breast Cancer.

    Clinical chemistry·2026
    Same journal

    In Reply to Reflexing NT-proBNP for sFlt-1/PlGF Ratios That Fall into the Measurement Uncertainty for Preeclampsia Risk Classification.

    Clinical chemistry·2026
    See all related articles

    This study demonstrates a flow-injection analysis system for detecting hydrogen peroxide using chemiluminescence. The method achieves high precision and sample throughput, suitable for various applications generating peroxide.

    Area of Science:

    • Analytical Chemistry
    • Chemical Kinetics
    • Biochemistry

    Background:

    • Flow-injection analysis (FIA) is a technique for automating wet chemical analysis.
    • Chemiluminescence detection offers high sensitivity for trace analysis.
    • Hydrogen peroxide is a common analyte in various industrial and biological processes.

    Purpose of the Study:

    • To evaluate a flow-injection analysis system for hydrogen peroxide detection using chemiluminescence.
    • To optimize system parameters for improved analytical performance.
    • To assess the system's applicability for real-time monitoring.

    Main Methods:

    • Utilized a sample-injection valve to introduce samples into a reagent stream.
    • Employed a luminol-based chemiluminescence reaction catalyzed by cupric ions.

    Related Experiment Videos

  • Investigated the impact of flow rate, sample volume, and reaction kinetics on the signal.
  • Measured chemiluminescent response using a photomultiplier detector.

    • Main Results:

    • Achieved precisions of 1-2% relative standard deviation for replicate measurements.
    • Demonstrated a sample throughput of up to six samples per minute with minimal peak overlap.
    • Characterized the effects of key parameters on response magnitude, duration, and repeatability.

    Conclusions:

    • The developed flow-injection analysis system provides a precise and efficient method for hydrogen peroxide determination.
    • The system's high sample throughput and adaptability make it suitable for continuous monitoring.
    • This detection approach can be integrated into processes involving peroxide generation.