Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inhibitory effects of mitomycin-related compounds lacking the C1-C2 aziridine ring.

C M Mercado, M Tomasz

    Antimicrobial Agents and Chemotherapy
    |January 1, 1972
    PubMed
    Summary

    Three mitomycin C derivatives kill bacteria by inhibiting DNA synthesis and cell division. These compounds demonstrate that the C(1)-C(2) aziridine ring is not essential for mitomycin C's bactericidal activity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Reductive activation of mitomycin A by thiols.

    Organic letters·2001
    Same author

    Solution structure of a guanine-N7-linked complex of the mitomycin C metabolite 2,7-diaminomitosene and DNA. Basis of sequence selectivity.

    Biochemistry·2001
    Same author

    Selective activation of mitomycin A by thiols to form DNA cross-links and monoadducts: biochemical basis for the modulation of mitomycin cytotoxicity by the quinone redox potential.

    Journal of medicinal chemistry·2001
    Same author

    Bioreductive metabolism of mitomycin C in EMT6 mouse mammary tumor cells: cytotoxic and non-cytotoxic pathways, leading to different types of DNA adducts. The effect of dicumarol.

    Biochemical pharmacology·2001
    Same author

    Structure of adduct X, the last unknown of the six major DNA adducts of mitomycin C formed in EMT6 mouse mammary tumor cells.

    Chemical research in toxicology·2000
    Same author

    Mitomycin C linked to DNA minor groove binding agents: synthesis, reductive activation, DNA binding and cross-linking properties and in vitro antitumor activity.

    Bioorganic & medicinal chemistry·2000

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Drug Discovery

    Background:

    • Mitomycin C is a potent antibiotic and anticancer agent.
    • The C(1)-C(2) aziridine ring of mitomycin C was thought to be crucial for its biological activity.
    • Understanding the mechanism of action of mitomycin C analogs is important for developing new antimicrobial agents.

    Purpose of the Study:

    • To investigate the biological activity of three novel mitomycin C derivatives.
    • To determine if the C(1)-C(2) aziridine ring is essential for the bactericidal effects of mitomycin C.

    Main Methods:

    • Bacterial cultures were treated with three mitomycin C derivatives.
    • Cell viability was assessed.
    • Deoxyribonucleic acid synthesis was measured.
    • Cell division was monitored.

    Main Results:

    • All three mitomycin C derivatives reduced bacterial viability.
    • These compounds inhibited deoxyribonucleic acid synthesis.
    • Cell division was also inhibited by the derivatives.
    • Importantly, none of the active derivatives possessed the C(1)-C(2) aziridine ring.

    Conclusions:

    • The C(1)-C(2) aziridine ring is not essential for the bactericidal activity of mitomycin C.
    • Novel mitomycin C derivatives can be developed with potent antimicrobial effects.
    • These findings expand our understanding of mitomycin C's mechanism of action.

    Related Experiment Videos