Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural proteins of Pichinde virus.

B A Ramos, R J Courtney, W E Rawls

    Journal of Virology
    |October 1, 1972
    PubMed
    Summary

    Pichinde virus, an arenovirus, has four protein components. Two are glycoproteins, V(II) and V(III), while V(I) and V(IV) are polypeptides, with V(III) identified as an envelope glycoprotein.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Automating behavioral analysis in neuroscience: Development of an open-source python software for more consistent and reliable results.

    Journal of neuroscience methods·2023
    Same author

    Spectral domain optical coherence tomography features in niacin maculopathy.

    Eye (London, England)·2014
    Same author

    Current state of medical-advice-seeking behaviour for symptoms of colorectal cancer: determinants of failure and delay in medical consultation.

    Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2012
    Same author

    Intracellular trafficking of the UL11 tegument protein of herpes simplex virus type 1.

    Journal of virology·2001
    Same author

    Membrane targeting properties of a herpesvirus tegument protein-retrovirus Gag chimera.

    Journal of virology·2000
    Same author

    Search for antiviral activity of certain medicinal plants from Córdoba, Argentina.

    Revista latinoamericana de microbiologia·2000

    Area of Science:

    • Virology
    • Molecular Biology
    • Biochemistry

    Background:

    • Pichinde virus belongs to the arenovirus family.
    • Understanding viral protein composition is crucial for studying viral structure and function.

    Purpose of the Study:

    • To characterize the polypeptide composition of Pichinde virus.
    • To investigate the functional roles of identified viral proteins.

    Main Methods:

    • Polyacrylamide gel electrophoresis was used to separate viral proteins.
    • Incorporation of glucosamine was analyzed to identify glycoproteins.
    • Treatment with Nonidet P-40 was employed to assess protein-nucleic acid associations.

    Main Results:

    • Four distinct polypeptides (V(I), V(II), V(III), V(IV)) were identified in Pichinde virus.
    • V(II) and V(III) were identified as glycopeptides due to glucosamine incorporation.
    • V(III) was removed by Nonidet P-40, suggesting it is an envelope glycoprotein.
    • V(I) and V(II) remained associated with viral nucleic acid, indicating ribonucleoprotein complexes.

    Conclusions:

    • Pichinde virus possesses a complex protein structure with distinct functional roles.
    • V(I) likely functions as a ribonucleoprotein component.
    • V(III) is identified as an envelope glycoprotein.
    • V(II) may serve dual roles as a membrane component and a nucleoprotein.

    Related Experiment Videos