Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Higher-order structures of chromatin in solution.

P Suau, E M Bradbury, J P Baldwin

    European Journal of Biochemistry
    |July 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Post-translational modifications of the intrinsically disordered terminal domains of histone H1: effects on secondary structure and chromatin dynamics.

    Chromosoma·2016
    Same author

    An improved double-beam infrared microspectrometer.

    Applied optics·2010
    Same author

    Orienting rigid and flexible biological assemblies in ferrofluids for small-angle neutron scattering studies.

    Biophysical journal·2009
    Same author

    [Recognition of internal (TTAGGG)n repeats by telomeric protein TRF1 and its role in maintenance of chromosomal stability in Chinese hamster cells].

    Tsitologiia·2004
    Same author

    Dephosphorylation of histone gamma-H2AX during repair of DNA double-strand breaks in mammalian cells and its inhibition by calyculin A.

    Radiation research·2003
    Same author

    Structure of the histone-core octamer in KCl/phosphate crystals at 2.15 A resolution.

    Acta crystallographica. Section D, Biological crystallography·2003
    Same journal

    Comparison of expression patterns and cell adhesion properties of the mouse biliary glycoproteins Bgp1 and Bgp2.

    European journal of biochemistry·2020
    Same journal

    AB 3.1.1.1 (or EC 3.1.1.?).

    European journal of biochemistry·2020
    Same journal

    Cdk5.

    European journal of biochemistry·2018
    Same journal

    Structure of the core oligosaccharide of a rough-type lipopolysaccharide of Pseudomonas syringae pv. phaseolicola.

    European journal of biochemistry·2004
    Same journal

    Monitoring ligand-mediated nuclear receptor-coregulator interactions by noncovalent mass spectrometry.

    European journal of biochemistry·2004
    Same journal

    Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy.

    European journal of biochemistry·2004
    See all related articles

    Neutron scatter studies reveal how chicken erythrocyte chromatin

    Area of Science:

    • Structural biology
    • Biophysics
    • Molecular genetics

    Background:

    • Chromatin, the complex of DNA and proteins, forms the basis of eukaryotic chromosomes.
    • Understanding chromatin structure is crucial for gene regulation and cellular processes.
    • The 10 nm nucleofilament represents the most basic level of DNA packaging within chromatin.

    Purpose of the Study:

    • To investigate the structural organization of chicken erythrocyte chromatin using neutron scatter.
    • To determine the parameters of the 10 nm nucleofilament and its structural transitions with varying ionic strength.
    • To model the arrangement of core particles within nucleofilaments and higher-order structures.

    Main Methods:

    • Neutron scatter studies on gently prepared chicken erythrocyte chromatin.

    Related Experiment Videos

  • Analysis of contrast dependence of cross-section radius of gyration (Rg).
  • Modeling of core particle arrangements within nucleofilaments and supercoils.

    • Main Results:

    • At low ionic strength, the 10 nm nucleofilament has a mass per unit length corresponding to one nucleosome per 8-12 nm.
    • Increasing ionic strength induces a transition to a second-order chromatin structure, with an abrupt increase in Rg.
    • A family of supercoils of nucleosomes is suggested, contracting with increasing ionic strength, with a hydrated supercoil diameter of 34 nm.

    Conclusions:

    • The arrangement of core particles in the 10 nm nucleofilament is edge-to-edge or with faces inclined within 20 degrees to the axis.
    • The transition to higher-order chromatin structure involves supercoiling of nucleosomes.
    • The study provides insights into the dynamic structural changes of chromatin with varying ionic conditions.