Solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to Mycoplasma pulmonis

A solid-phase radioimmunoassay (RIA) was used for the detection and quantitation of immunoglobulin class-specific antibodies to Mycoplasma pulmonis in mouse sera. The RIA was found to be more sensitive than the single radial haemolysis (SRH) test for detecting antibodies in sera from mice at intervals after inoculation with M. pulmonis, since antibody was detected in 26 out of 29 serum samples by RIA but in only 8 out of 29 samples by SRH. A method is described for quantitating immunoglobulin class-specific antibody in test sera by reference to a standard serum. The RIA should be useful for the study of the relative importance of various immunoglobulin classes in resistance to mycoplasma infections.