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Related Experiment Videos

Opiate receptor: demonstration in nervous tissue.

C B Pert, S H Snyder

    Science (New York, N.Y.)
    |March 9, 1973
    PubMed
    Summary

    Tritiated naloxone specifically binds to opiate receptors in mammalian brain and guinea pig intestine. This binding confirms the opiate receptor

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    Area of Science:

    • Pharmacology
    • Neuroscience
    • Biochemistry

    Background:

    • Opiate receptors are crucial targets for pain management and addiction therapies.
    • Understanding the precise localization and binding characteristics of these receptors is essential.

    Purpose of the Study:

    • To investigate the specific binding of tritiated naloxone to opiate receptors.
    • To determine the distribution of opiate receptors in mammalian tissues.
    • To correlate receptor binding with the pharmacological potency of opiates and antagonists.

    Main Methods:

    • Utilized radioligand binding assays with tritiated naloxone.
    • Examined binding in mammalian brain tissue and guinea pig intestine preparations.
    • Performed competition binding studies with various opiate agonists and antagonists.

    Main Results:

    • Tritiated naloxone demonstrated specific binding to opiate receptors in both mammalian brain and guinea pig intestine.
    • The competition for receptor binding by different opiates and antagonists accurately reflected their known pharmacological potencies.
    • Opiate receptor binding was exclusively observed in nervous tissue.

    Conclusions:

    • Tritiated naloxone is a valuable tool for specifically labeling and studying opiate receptors.
    • The findings confirm the presence of pharmacologically relevant opiate receptors in mammalian brain and peripheral nervous tissue.
    • Opiate receptors are localized within nervous tissue.

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