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Characterization of human B lymphocyte specific antigens
Journal of Immunology (Baltimore, Md. : 1950)
|January 1, 1975
Summary
This study developed anti-human B cell serum (ABS) effective against chronic lymphocytic leukemia (CLL) cells. ABS demonstrated specificity for B cells, concentrating in lymphoid follicles and germinal centers.
Area of Science:
- Immunology
- Hematology
Background:
- Rabbit anti-human tonsil serum (AHTS) was used as a starting point.
- Sequential absorption aimed to achieve B cell specificity.
Purpose of the Study:
- To develop and characterize an anti-human B cell serum (ABS).
- To confirm the B cell specificity of the developed serum.
Main Methods:
- Sequential absorption of rabbit anti-human tonsil serum (AHTS) with various human cells and tissues.
- Testing ABS cytotoxicity against chronic lymphocytic leukemia (CLL) cells, thymocytes, and lymphocytes.
- Indirect immunofluorescence on human tonsil and lymph node tissue sections.
Main Results:
- ABS lysed the majority of CLL cells and some lymphocytes, but was nontoxic to thymocytes.
- ABS activity was retained after absorption with non-B cell tissues but reduced by absorption with tonsil and CLL cells.
- Immunofluorescence showed ABS-reactive cells concentrated in lymphoid follicles and germinal centers, sparing plasma cells and thymus-dependent areas.
Conclusions:
- The developed serum (ABS) exhibits specificity for human B cells.
- ABS is a potential tool for targeting B cells, particularly in conditions like CLL.