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Related Experiment Videos

11-cis-Retinal-binding protein from bovine retina. Isolation and partial characterization.

G W Stubbs, J C Saari, S Futterman

    The Journal of Biological Chemistry
    |September 10, 1979
    PubMed
    Summary
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    Researchers purified bovine 11-cis-retinal-binding protein, identifying endogenous 11-cis-retinol and 11-cis-retinal ligands. This protein

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Vision Science

    Background:

    • The visual cycle involves the regeneration of 11-cis-retinal, a crucial chromophore for photoreceptor proteins.
    • Understanding the proteins involved in retinal binding and transport is essential for elucidating visual cycle mechanisms.

    Purpose of the Study:

    • To purify and characterize the 11-cis-retinal-binding protein from bovine retina.
    • To investigate the endogenous ligands associated with the purified binding protein.
    • To assess the protein's relationship with opsin and its potential role in the visual cycle.

    Main Methods:

    • Purification using gel filtration, ion exchange, and hydroxylapatite chromatography.
    • Molecular weight determination via SDS-PAGE and gel filtration.

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  • Spectroscopic analysis (UV-Vis) to identify bound ligands.
  • Immunological cross-reactivity tests with anti-bovine opsin antibodies.
  • Amino acid composition analysis.

    • Main Results:

    • The 11-cis-retinal-binding protein was purified to homogeneity with molecular weights of 33,000 (SDS-PAGE) and 36,000 (gel filtration).
    • The purified protein exhibited absorbance maxima at 340 nm and 425 nm, attributed to bound 11-cis-retinol and 11-cis-retinal, respectively.
    • The protein did not cross-react with anti-bovine opsin antibodies and had a distinct amino acid composition.
    • Retinal-binding protein was found at approximately 1 nmol/retina or 1 mol per 20 mol of opsin.

    Conclusions:

    • The purified protein is a distinct entity from opsin, tentatively identified as 11-cis-retinal-binding protein.
    • The presence of endogenous 11-cis-retinol and 11-cis-retinal suggests a significant role in the visual cycle.
    • Its specificity and abundance indicate a major function in the transport or storage of retinoids within the retina.