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Macrophage migration technique using coccidioidin
Infection and Immunity
|February 1, 1973
Abstract:
Guinea pigs sensitized by repeated skin testing with potent, merthiolated coccidioidin, equal to Smith standard, exhibited erythema, edema, and an induration-like reaction to intradermal injection of the preparation. No statistically significant migration inhibition of peritoneal exudate macrophages from these animals was observed.
Insights
Guinea pigs showed skin reactions after coccidioidin testing. However, their macrophages did not exhibit significant migration inhibition, suggesting a complex immune response to coccidioidin.
Area of Science:
- Immunology
- Dermatology
- Microbiology
Background:
- Coccidioidin is used to test for Coccidioides immitis infection.
- Skin testing is a common method for assessing cell-mediated immunity.
Purpose of the Study:
- To evaluate the immune response in guinea pigs sensitized to coccidioidin.
- To investigate both dermal hypersensitivity and macrophage migration inhibition.
Main Methods:
- Guinea pigs were repeatedly skin tested with merthiolated coccidioidin.
- Intradermal injections were administered to assess skin reactions.
- Peritoneal exudate macrophages were isolated to test for migration inhibition.
Main Results:
- Sensitized guinea pigs displayed erythema, edema, and induration upon intradermal injection.
- No statistically significant migration inhibition of macrophages was observed in these animals.
Conclusions:
- Guinea pigs exhibit a dermal hypersensitivity reaction to coccidioidin.
- The observed skin reactions do not correlate with significant macrophage migration inhibition in this model.