Application of the parallel line bioassay method to quantitative determination of the HBs antigen in radioimmunoassay

Precise and reproducible quantitative determination of hepatitis B surface antigen by applying the parallel line bioassay method was proposed. In this method, the value of any given sample can be expressed in terms of a relative value to a fixed standard or reference preparation. Decrease in antigenic reactivity due to the lowered protein content could be prevented by using 0.02% human albumin in physiological saline solution as the diluent. The use of such stabilizer(s) should be considered in the dilution or storage of samples containing HBs antigen.