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Related Experiment Videos

Cytidylic acid "a" trihydrate: structure and conformation.

G Kartha, G Ambady, M A Viswamitra

    Science (New York, N.Y.)
    |February 2, 1973
    PubMed
    Summary

    Cytidylic acid "a", or cytidine 2'-monophosphate, was analyzed using X-ray crystallography. The study revealed specific molecular conformations and hydrogen bonding critical for crystal structure.

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    Area of Science:

    • Biochemistry
    • Crystallography
    • Molecular Biology

    Background:

    • Cytidylic acid "a" (cytidine 2 -monophosphate) is a fundamental nucleotide.
    • Understanding nucleotide structure is crucial for molecular biology and drug design.

    Purpose of the Study:

    • To determine the crystal structure of cytidylic acid "a" (cytidine 2 -monophosphate).
    • To elucidate the molecular conformation and intermolecular interactions within the crystal lattice.

    Main Methods:

    • X-ray diffraction analysis was performed on cytidylic acid "a" crystals.
    • The crystal structure was solved in the triclinic space group P1.

    Main Results:

    • The crystal unit cell contains two cytidine monophosphate molecules and six water molecules.
    • Both cytidine monophosphate molecules exhibit an anti base conformation.
    • The ribose ring adopts a C(2 )-endo puckered form, and the O(5 ) hydroxyl group is gauche-gauche.
    • A short hydrogen bond links the two molecules via their phosphate oxygens.

    Conclusions:

    • The study provides detailed structural information on cytidine 2 -monophosphate.
    • The observed conformation and hydrogen bonding are key to the crystal packing.

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