Effect of vinblastine on the chemotactic responsiveness of normal human neutrophils

The effect of vinblastine on the mobility of human neutrophils was studied. Chemotactic responsiveness was decreased by approximately 80 to 95%, as measured in Boyden chambers, whereas random mobility, as measured in glass capillary tubes, was approximately doubled. Chemotactic responsiveness could be partially restored after dialysis of the treated cells, which is not compatible with an action of vinblastine on microtubules. The possibility is considered that this observation is an additional example of the effect of several microtubular binding agents on phenomena mediated at the phagocytic surface.