Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intermediates in fatty acid oxidation.

H B Stewart, P K Tubbs, K K Stanley

    The Biochemical Journal
    |January 1, 1973
    PubMed
    Summary
    This summary is machine-generated.

    Mitochondrial extracts and intact mitochondria break down fatty acids, forming acetyl-CoA through intermediate molecules like octanoyl-CoA. This process involves free intermediates rather than a single enzyme complex.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evaluation of multiplex tandem PCR (MT-PCR) assays for the detection of bacterial resistance genes among Enterobacteriaceae in clinical urines.

    The Journal of antimicrobial chemotherapy·2018
    Same author

    Constructing expression cDNA libraries using unphosphorylated adaptors.

    Methods in molecular biology (Clifton, N.J.)·2011
    Same author

    Expression Screening of cDNA Libraries in pEX.

    Methods in molecular biology (Clifton, N.J.)·2011
    Same author

    Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.

    Methods in molecular biology (Clifton, N.J.)·2011
    Same author

    Epitope Mapping Using pEX.

    Methods in molecular biology (Clifton, N.J.)·2011
    Same author

    Interaction of caveolin with 7-ketocholesterol.

    Atherosclerosis·2001

    Area of Science:

    • Biochemistry
    • Cellular Metabolism
    • Mitochondrial Function

    Background:

    • Mitochondria are crucial for cellular energy production, primarily through fatty acid oxidation.
    • The precise mechanisms and intermediates involved in fatty acyl-CoA degradation within mitochondria are complex and actively researched.

    Purpose of the Study:

    • To investigate the pathway of fatty acyl-CoA conversion to acetyl-CoA in liver and kidney mitochondria.
    • To identify and characterize the intermediate compounds formed during octanoyl-CoA oxidation.

    Main Methods:

    • Utilized aqueous extracts of acetone-dried liver and kidney mitochondria.
    • Employed radiolabeled octanoyl-CoA ([1-(14)C] and [8-(14)C]) to trace metabolic flow.
    • Applied the hydroxamate technique to identify CoA-ester intermediates during fatty acid oxidation.

    Related Experiment Videos

    Main Results:

    • Aqueous extracts efficiently converted fatty-acyl-CoA to acetyl-CoA, with label appearing rapidly in acetyl-CoA.
    • Hydroxamate analysis revealed intermediates including 3-hydroxyoctanoyl-CoA, hexanoyl-CoA, and butyryl-CoA, suggesting a stepwise degradation.
    • Intact mitochondria also produced intermediates, but accumulated significantly more octanoyl-CoA compared to other intermediates.

    Conclusions:

    • Mitochondrial fatty acid oxidation proceeds via the formation of free intermediates, rather than a single, uninterrupted multienzyme complex.
    • The hydroxamate technique is effective for identifying CoA-ester intermediates in mitochondrial metabolism.
    • Differences in intermediate accumulation between extracts and intact mitochondria suggest compartmentation or regulatory mechanisms.