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Triglyceride lipase activity and human platelets.

K Lote, C Lowery

    Thrombosis and Haemostasis
    |April 23, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Platelet-rich plasma shows higher triglyceride lipase activity, including lipoprotein lipase (LPL) and hepatic triglyceride lipase (H-TGL), compared to platelet-poor plasma. Platelets contribute minimally to this activity and have distinct lipase characteristics.

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    Area of Science:

    • Biochemistry
    • Hematology
    • Lipid Metabolism

    Background:

    • Triglyceride lipases are crucial enzymes in lipid metabolism.
    • Their activity in human plasma is influenced by various blood components.
    • Understanding lipase contribution from different blood cells is important for metabolic research.

    Purpose of the Study:

    • To investigate the role of platelets in postheparin plasma triglyceride lipase activity.
    • To differentiate the contributions of lipoprotein lipase (LPL) and hepatic triglyceride lipase (H-TGL) from platelets.

    Main Methods:

    • Comparison of triglyceride lipase activity in platelet-rich versus platelet-poor human postheparin plasma.
    • Gel filtration chromatography (Sepharose 2 B) to separate platelets from plasma.

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  • Enzyme activity assays with NaCl and specific antibodies (anti-LPL, anti-H-TGL).

    • Main Results:

    • Significantly higher total, LPL, and H-TGL activities were observed in platelet-rich plasma.
    • Gel filtration successfully separated platelets, revealing minimal lipase activity associated with isolated platelets.
    • Platelet-associated lipase activity showed inhibition by high salt and anti-H-TGL antibody, but not anti-LPL antibody.

    Conclusions:

    • Platelets contribute to the overall triglyceride lipase activity in human postheparin plasma.
    • The lipase activity associated with platelets is primarily hepatic triglyceride lipase (H-TGL) and is salt-sensitive.
    • Lipoprotein lipase (LPL) activity is predominantly non-platelet derived in this context.