Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Catecholamine uptake in cerebral cortex: adaptive change induced by fighting.

E D Henley, B Moisset, B L Welch

    Science (New York, N.Y.)
    |June 8, 1973
    PubMed
    Summary

    Fighting stress alters norepinephrine (NE) brain uptake. Chronic attack lowers NE affinity and increases uptake sites, while acute fights also reduce NE affinity and d-amphetamine sensitivity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Comparison of ponderosa pines as feed and nonfeed trees for abert squirrels.

    Journal of chemical ecology·2013
    Same author

    Monoterpenoid content of sage grouse ingesta.

    Journal of chemical ecology·2013
    Same author

    The generalisation of student's problems when several different population variances are involved.

    Biometrika·2010
    Same author

    The hospital. Psychology's challenge in the 1990's.

    The American psychologist·1990
    Same author

    The Rand Health Insurance Study: a summary critique.

    Medical care·1987
    Same author

    Psychology and HMOs. New partnership or new adversary?

    The American psychologist·1985

    Area of Science:

    • Neuroscience
    • Behavioral Neuroscience
    • Neuropharmacology

    Background:

    • The catecholamine uptake process in the brain is crucial for neurotransmitter regulation.
    • Stress, particularly social stress like fighting, can significantly impact neurochemical processes.

    Purpose of the Study:

    • To investigate the effects of fighting-induced stress on the kinetics of catecholamine uptake in the brain.
    • To determine how chronic and acute fighting influences norepinephrine (NE) uptake and its inhibition by d-amphetamine.

    Main Methods:

    • Assessing the kinetics of norepinephrine uptake in cerebral cortical homogenates from mice subjected to different fighting paradigms.
    • Measuring apparent Michaelis constant (Km) and maximum velocity (Vmax) for NE uptake.
    • Determining the inhibition constant (Ki) for d-amphetamine inhibition of NE uptake.

    Main Results:

    • Group-caged mice under chronic attack showed increased Km for NE uptake and increased Ki for d-amphetamine inhibition, indicating lowered affinity.
    • Male mice after acute intense fights exhibited increased Km and Vmax for NE uptake, suggesting reduced affinity and more uptake sites.
    • These changes in NE uptake kinetics were observed 18-20 hours post-fight.

    Conclusions:

    • Social stress from fighting alters norepinephrine reuptake in the cerebral cortex.
    • Fighting stress leads to decreased affinity for norepinephrine reuptake and an increase in the number of available uptake sites.
    • Stress-induced changes also result in a lowered affinity for d-amphetamine inhibition of norepinephrine uptake.

    Related Experiment Videos