Replication process of the parvovirus H-1. I. Kinetics in a parasynchronous cell system

Parasynchronous cultures of hamster embryo cells were used to study some of the events in the replication process of the parvovirus H-1. Synthesis of viral DNA, viral hemagglutinating antigen, and infectious virus were examined. It was found that initiation of DNA synthesis, on which subsequent viral hemagglutinin synthesis was dependent, occurred at a specific time in late S-phase. Production of H-1 viral protein was shown to be sensitive to inhibition by alpha-amanitin. The implications of these findings are discussed.