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Related Experiment Videos

Quality control of agar diffusion susceptibility tests.

R C Knowles, T D Moore

    American Journal of Clinical Pathology
    |August 1, 1979
    PubMed
    Summary
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    The College of American Pathologists Quality Assurance Service analyzed over 2.3 million antibiotic determinations. Data from the Bauer-Kirby method often exceeded established precision and accuracy control limits.

    Area of Science:

    • Clinical Microbiology
    • Pharmaceutical Quality Control

    Background:

    • The College of American Pathologists (CAP) Microbiology Program has provided quality assurance since 1974.
    • Over 180 laboratories have participated, generating extensive data on antibiotic susceptibility testing.
    • This study analyzes a significant dataset of antibiotic determinations on reference strains.

    Purpose of the Study:

    • To evaluate the performance of antibiotic susceptibility testing methods within a large-scale quality assurance program.
    • To compare current quality assurance data against established precision and accuracy control limits.
    • To identify trends and potential areas for improvement in antibiotic testing methodologies.

    Main Methods:

    • Analysis of 2,372,000 antibiotic determinations submitted to the CAP Quality Assurance Service (QAS).

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  • Primary method analyzed: standard Bauer-Kirby method (89.5% of data).
  • Secondary method analyzed: agar overlay modification by Barry et al. (8.4% of data).

    • Main Results:

    • Standard statistical analysis was performed for each antimicrobial/reference strain combination using the Bauer-Kirby method.
    • Quality Assurance Service (QAS) data were compared to historical precision and accuracy control limits from collaborative studies.
    • A significant proportion of QAS data exceeded existing control limits, indicating potential variability or shifts in performance.

    Conclusions:

    • The extensive data from the CAP QAS highlights the performance of antibiotic susceptibility testing on a national scale.
    • Exceedance of established control limits suggests a need to review and potentially update current performance benchmarks.
    • Continuous quality assessment is crucial for maintaining accuracy and reliability in clinical microbiology antibiotic testing.