Comparative lethal effects on mice of ruminal fluid from cattle fed hay or grain

Strained ruminal fluid from cattle fed hay or grain was lethal to mice when injected intraperitoneally, but the fluid from grain-fed cattle was approximately 3.7 times more toxic than that from hay-fed cattle. The lethal factor(s) was not resistant to heat, was nondialyzable, was retained on Seitz and membrane filters, and was associated with the bacterial fraction of ruminal fluid. We concluded that death of the mice resulted from infection produced by facultative bacteria normally in ruminal fluid. Ruminal fluid from grain-fed cattle contained a greater number of facultative bacteria than did that from hay-fed cattle.