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["In vitro" interactions between influenza virus and mouse lung alveolar macrophages (author's transl)]
Abstract:
Interactions between influenza virus A/PR/8/34 (H0N1) and Balb/c mouse lung alveolar macrophages have been studied in vitro. One day after initiation of alveolar macrophage culture in 35 mm Falcon dishes, the virus suspension was allowed to adsorb to the cells for 1 h. Detachment of cells from the plastic substrate, morphological changes in adherent cells and decreased phagocytosis of heat-killed Candida albicans occured slowly as compared to control cultures. These facts appeared to be directly correlated to the concentration of viruses in the inoculum. Data yielded by virus titrations, electron microscopy and immunofluorescence suggest that mouse lung alveolar macrophages are able to take up a large amount of viral particles and inhibit their replication, allowing only an abortive viral cycle.
Insights
Mouse lung alveolar macrophages interact with influenza A virus. Macrophages internalize many virus particles, inhibiting viral replication and leading to an abortive viral cycle.
Area of Science:
- Immunology
- Virology
Context:
- In vitro study of host-pathogen interactions.
- Utilizes Balb/c mouse lung alveolar macrophages and influenza virus A/PR/8/34 (H0N1).
Purpose:
- To investigate the in vitro interaction between influenza virus and mouse alveolar macrophages.
- To characterize the cellular and viral responses following infection.
Summary:
- Alveolar macrophages were cultured and exposed to influenza virus.
- Observed slow detachment, morphological changes, and decreased phagocytosis correlated with virus concentration.
- Virus titrations, electron microscopy, and immunofluorescence indicated macrophages internalized significant viral loads.
- Macrophages inhibited viral replication, resulting in an abortive viral cycle.
Impact:
- Provides insights into the innate immune response of lung macrophages to influenza virus.
- Suggests a mechanism of viral control at the macrophage level.
- Informs understanding of early host-virus interactions in the respiratory tract.