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Raman spectroscopic evidence for two conformations of uncomplexed valinomycin in the solid state
Science (New York, N.Y.)
|October 26, 1973
Summary
Raman spectroscopy reveals distinct molecular conformations for valinomycin, a carrier transport molecule. Hydrogen bonding influences the structure of valinomycin crystals, as evidenced by spectral changes.
Area of Science:
- Molecular Spectroscopy
- Biophysical Chemistry
Background:
- Valinomycin is a crucial carrier transport molecule.
- Understanding its conformations is key to its function.
- Raman spectroscopy offers vibrational insights into molecular structure.
Purpose of the Study:
- To investigate the different conformations of valinomycin.
- To apply Raman spectroscopy for structural elucidation.
- To explore the impact of crystal growth solvents on valinomycin structure.
Main Methods:
- Raman spectroscopy was employed.
- Valinomycin crystals were grown from n-octane, acetone, and o-dichlorobenzene.
- Vibrational spectra were analyzed to identify conformational differences.
Main Results:
- Splitting of ester and amide carbonyl stretch vibrations was observed in Raman spectra of valinomycin grown from n-octane and acetone.
- This spectral feature suggests intramolecular hydrogen bonding in ester carbonyl groups.
- Valinomycin grown from o-dichlorobenzene did not exhibit this hydrogen bonding signature.
Conclusions:
- Raman spectroscopy successfully differentiated valinomycin conformations.
- Intramolecular hydrogen bonding is present in specific crystal forms of valinomycin.
- Solvent choice during crystallization significantly impacts valinomycin's molecular conformation.