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The binding of urate by plasma proteins.
The Australian Journal of Experimental Biology and Medical Science
|February 1, 1979
Summary
Urate protein binding is relevant to gout pathogenesis. This study used an ultrafiltration method under physiological conditions, finding urate binding varied between sexes and didn't correlate with albumin or urate levels.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Pathophysiology
Background:
- Protein binding of urate is implicated in gout pathogenesis.
- In vitro studies face challenges in methodology and physiological relevance.
- Understanding urate-protein interactions is crucial for gout research.
Purpose of the Study:
- To accurately determine urate binding to proteins under physiological conditions.
- To investigate the relationship between urate binding, albumin, and total urate concentration.
- To explore factors influencing urate-protein interactions in normal individuals.
Main Methods:
- Urate binding was quantified using an ultrafiltration procedure.
- All experimental manipulations were conducted anaerobically at 37°C to mimic physiological conditions.
- Measurements were performed on samples from normal males and females.
Main Results:
- Normal urate binding was observed at 15% +/- 7.5% in males and 10% +/- 6.6% in females.
- Urate binding showed no significant correlation with albumin concentration.
- Urate binding did not significantly correlate with total urate concentration.
Conclusions:
- Urate-protein interaction is influenced by factors beyond free urate and binding protein concentrations.
- The described ultrafiltration technique offers a reliable method for studying urate binding.
- This methodology can be applied to investigate urate binding in pathological conditions like gout.