Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromosome studies on a human liposarcoma cell line.

E Bey, R Bernstein

    Cytogenetics and Cell Genetics
    |January 1, 1979
    PubMed
    Summary

    This study analyzed chromosome changes in a human liposarcoma cell line. Researchers found a stable hypodiploid mode, with a near-triploid stemline emerging under specific conditions, indicating significant chromosomal instability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Precision Measurement of Neutrino Oscillation Parameters with 10 Years of Data from the NOvA Experiment.

    Physical review letters·2026
    Same author

    Dual-Baseline Search for Active-to-Sterile Neutrino Oscillations in NOvA.

    Physical review letters·2025
    Same author

    Search for CP-Violating Neutrino Nonstandard Interactions with the NOvA Experiment.

    Physical review letters·2024
    Same author

    Technological advances in diabetes.

    British dental journal·2024
    Same author

    [Current role of the latissimus dorsi flap in traumatology: Analysis of the activity of a plastic surgery department in a military hospital].

    Annales de chirurgie plastique et esthetique·2023
    Same author

    [Pre-iliac tumor mass syndrome: An atypical rheumatoid nodule].

    Annales de chirurgie plastique et esthetique·2023

    Area of Science:

    • Cytogenetics
    • Cancer Biology
    • Molecular Oncology

    Background:

    • Liposarcoma is a common soft tissue sarcoma.
    • Understanding the chromosomal aberrations in liposarcoma cell lines is crucial for cancer research.
    • Cell line maintenance can influence observed karyotypic profiles.

    Purpose of the Study:

    • To characterize the numerical and structural chromosomal abnormalities in a human retroperitoneal liposarcoma cell line.
    • To investigate the impact of cell culture conditions on karyotypic stability.
    • To identify specific marker chromosomes and translocations associated with this liposarcoma.

    Main Methods:

    • Numerical and structural chromosome analysis using trypsin-Giemsa staining.
    • Karyotyping of liposarcoma cells under standard and extended culture conditions.
    • Quantitation of genetic material loss per cell spread.

    Main Results:

    • A stable hypodiploid mode was observed in standard cell culture.
    • A near-triploid stemline emerged when cells were not trypsinized, often a duplication of the hypodiploid mode.
    • Significant chromosomal instability was noted, with loss of homologs and formation of new marker chromosomes.
    • A consistent translocation between chromosomes 4 and 11 was identified as a key marker chromosome.
    • Quantitation revealed a mean genetic material loss of 12.0 +/- 3.7% per spread.

    Conclusions:

    • The retroperitoneal liposarcoma cell line exhibits significant karyotypic instability.
    • Cell culture conditions, specifically trypsinization frequency, can influence the emergence of different stemlines.
    • Specific chromosomal rearrangements, like the 4;11 translocation, may serve as diagnostic markers for this type of liposarcoma.

    Related Experiment Videos