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Presence of viruses in a strain of mycoplasma pulmonis
Abstract:
Filtrates prepared from heavily grown agar cultures of M. pulmonis strain Negroni-52 formed plaques on lawns of A. laidlawii strain JAl but not on those of M. pulmonis strains Ash or Negroni-52. The plaque-forming agent proved to be rod-shaped particles morphologically identical with mycoplasmavirus group 1. Evidence supporting the conclusion that the virus originated from Negroni-52 has been obtained. Electron microscopy revelaed that Negroni-52 is also a carrier of long-tailed phage-like particles.
Insights
Mycoplasmavirus group 1 from M. pulmonis strain Negroni-52 formed plaques on A. laidlawii, but not other M. pulmonis strains. This virus, identical to Negroni-52
Area of Science:
- Microbiology
- Virology
Background:
- Mycoplasma pulmonis is a common respiratory pathogen.
- Mycoplasmaviruses are viruses that infect Mycoplasma species.
Purpose of the Study:
- To investigate the presence and activity of mycoplasmaviruses in M. pulmonis strains.
- To characterize the plaque-forming agent found in M. pulmonis strain Negroni-52 filtrates.
Main Methods:
- Agar culture filtrates of M. pulmonis strain Negroni-52 were prepared.
- Plaque assays were performed on lawns of A. laidlawii and other M. pulmonis strains.
- Electron microscopy was used to examine the morphology of the plaque-forming agent.
Main Results:
- Filtrates from M. pulmonis strain Negroni-52 formed plaques on A. laidlawii lawns.
- No plaques were observed on lawns of M. pulmonis strains Ash or Negroni-52.
- The plaque-forming agent was identified as rod-shaped particles morphologically identical to mycoplasmavirus group 1.
- Electron microscopy revealed Negroni-52 also harbors long-tailed phage-like particles.
Conclusions:
- Mycoplasmavirus group 1 is present in M. pulmonis strain Negroni-52.
- This virus can infect and form plaques on A. laidlawii.
- M. pulmonis strain Negroni-52 may serve as a source of mycoplasmavirus group 1.