Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chemotactic factor inactivator release from rat leukocytes.

J D Hunt, P A Ward

    Inflammation
    |July 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Theory-Driven Interventions in Psychoneuroimmunology and HIV-1 Infection.

    CNS spectrums·2008
    Same author

    Lipid messengers as targets for antiangiogenic therapy.

    Current pharmaceutical design·2001
    Same author

    Suppression of tumor cell growth both in nude mice and in culture by n-3 polyunsaturated fatty acids: mediation through cyclooxygenase-independent pathways.

    Cancer research·2001
    Same author

    KRAS mutations are not predictive for progression of preneoplastic gastric lesions.

    Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2001
    Same author

    Orthotopic human lung carcinoma xenografts in BALB/c mice immunosuppressed with anti-CD4 monoclonal antibodies and chronic alcohol consumption.

    Cancer·2000
    Same author

    Evidence for autocrine actions of neuromedin B and gastrin-releasing peptide in non-small cell lung cancer.

    Pulmonary pharmacology & therapeutics·1999
    Same journal

    Correction to: Aberrant Accumulation of Cell-Free DNA Activates the cGAS-STING-TBK1 Pathway of γδ T Cells to Promote the Inflammatory Responses in Oral Lichen Planus.

    Inflammation·2026
    Same journal

    Baricitinib Inhibits the Aggressive Phenotype of Synovial Fibroblasts in Rheumatoid Arthritis via the JAK-STAT3-EGR1 Axis.

    Inflammation·2026
    Same journal

    Mitophagy in Metabolic Dysfunction-Associated Fatty Liver Disease: Mechanisms, Regulatory Networks, and Therapeutic Perspectives.

    Inflammation·2026
    Same journal

    A Self-Developed IL-6Rα Nanobody Alleviates Experimental Autoimmune Uveitis by Inhibiting Th1 and Th17 Cells.

    Inflammation·2026
    Same journal

    Dehydrocostus Lactone Activates Nrf2 Signaling Pathway to Attenuate Oxidative Stress, Inflammation, and Excessive Autophagy in a Mouse Model of Chronic Obstructive Pulmonary Disease.

    Inflammation·2026
    Same journal

    Single-cell and Spatial Transcriptomic Profiling Reveal that LAPTM5-mediated Ferroptosis in Macrophages Induces Fibroblast Dysfunction and Amplifies Periodontal Inflammation.

    Inflammation·2026
    See all related articles

    Rat leukocytes release chemotactic factor inactivator (CFI) during phagocytosis, which helps regulate inflammatory responses by neutralizing immune cell attractants.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Leukocytes, including neutrophils and macrophages, play a crucial role in inflammatory responses.
    • Chemotactic factors attract immune cells to sites of infection or injury.
    • Regulation of inflammatory mediators is essential to prevent excessive tissue damage.

    Purpose of the Study:

    • To investigate the release of chemotactic factor inactivator (CFI) by rat leukocytes during phagocytosis.
    • To characterize the nature of CFI activity and its targets.
    • To explore the potential role of CFI in regulating inflammatory responses.

    Main Methods:

    • Incubation of rat neutrophils, alveolar macrophages, and peritoneal macrophages with opsonized zymosan particles and immune complexes.
    • Assay of supernatant fluids and leukocyte homogenates for CFI activity against various chemotactic factors.

    Related Experiment Videos

  • Measurement of beta-glucuronidase as a marker for leukocyte activation.

    • Main Results:

    • Phagocytizing leukocytes released CFI activities targeting complement-derived (C3, C5) and bacterial chemotactic factors.
    • CFI activity was detected in both supernatant fluids and disrupted leukocyte homogenates.
    • CFI release was dependent on incubation time with phagocytic stimuli.
    • No direct correlation was found between CFI activity and beta-glucuronidase release.

    Conclusions:

    • Leukocytes actively release CFI during phagocytosis, suggesting a self-regulatory mechanism.
    • CFI inactivates key chemotactic factors, potentially limiting the inflammatory response.
    • This mechanism may serve as a crucial "turn-off" signal to control inflammation.