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Infection of chicken erythrocytes with influenza and other viruses
Abstract:
Chicken erythrocytes can be infected by the fowl plague (Rostock) strain (FP/R) of influenza type A, Newcastle disease virus (NDV), and Semliki Forest virus (SFV). Only NDV and SFV produced infectious progeny, albeit at low levels. Infection by FP/R was monitored by de novo synthesis of viral proteins, and the proteins synthesized could be identified by comparison with infected chicken fibroblast cells. FP/R synthesized far greater amounts of viral protein than did NDV or SFV.
Insights
Chicken erythrocytes can be infected by fowl plague (FP/R) influenza A, Newcastle disease virus (NDV), and Semliki Forest virus (SFV). FP/R synthesized significantly more viral proteins than NDV or SFV, though only NDV and SFV produced infectious progeny.
Area of Science:
- Virology
- Cellular Biology
- Infectious Diseases
Background:
- Erythrocytes, or red blood cells, are a common target for various viruses.
- Understanding viral replication and protein synthesis in different host cells is crucial for disease control.
Purpose of the Study:
- To investigate the susceptibility of chicken erythrocytes to infection by fowl plague (Rostock) strain (FP/R) influenza A, Newcastle disease virus (NDV), and Semliki Forest virus (SFV).
- To compare the viral protein synthesis and progeny production across these three viral infections in chicken erythrocytes.
Main Methods:
- Infection of chicken erythrocytes with FP/R, NDV, and SFV.
- Monitoring of de novo viral protein synthesis.
- Identification of synthesized viral proteins by comparison with infected chicken fibroblast cells.
Main Results:
- Chicken erythrocytes were susceptible to infection by FP/R, NDV, and SFV.
- NDV and SFV produced low levels of infectious progeny.
- FP/R synthesized significantly greater amounts of viral protein compared to NDV and SFV.
Conclusions:
- Chicken erythrocytes support viral infection and protein synthesis, with varying outcomes for progeny production.
- Influenza A (FP/R) demonstrates a higher capacity for viral protein synthesis in chicken erythrocytes than NDV or SFV.
- Further research is needed to elucidate the mechanisms behind differential progeny production and protein synthesis in virus-infected erythrocytes.