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Methods for determinging carbohydrates, hydroxymethylfurfural, and proline in honey: collaborative study.

Journal - Association of Official Analytical Chemists

|May 1, 1979

Summary

A modified honey carbohydrate analysis method was validated and adopted by AOAC. High-pressure liquid chromatography also proved precise for honey sugar analysis and was adopted.

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Area of Science:

Food chemistry
Analytical chemistry
Agricultural science

Background:

Official methods for honey carbohydrate analysis require validation and improvement for accuracy.
Accurate quantification of honey sugars (monosaccharides, disaccharides, higher sugars) is crucial for quality control.

Purpose of the Study:

To collaboratively study a modified selective absorption method for honey carbohydrate determination.
To evaluate high-pressure liquid chromatography (HPLC) for honey sugar analysis.
To assess methods for hydroxymethylfurfural and proline determination in honey.

Main Methods:

Collaborative study of a modified selective absorption method for honey carbohydrates.
High-pressure liquid chromatography (HPLC) analysis of glucose, fructose, and sucrose in honey.
Evaluation of specific methods for hydroxymethylfurfural and proline.

Main Results:

The modified selective absorption method provided satisfactory determinations of sucrose, total monosaccharides, disaccharides, and higher sugars.
HPLC demonstrated superior precision and concordant results for glucose, fructose, and sucrose compared to the modified official method.
Two hydroxymethylfurfural methods were not suitable; a proline method was adopted.

Conclusions:

The modified selective absorption method for honey carbohydrates was adopted by AOAC.
HPLC is a precise and reliable method for analyzing key sugars in honey.
Standardized methods for proline analysis in honey were established.

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