Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biosynthesis of monensin.

L E Day, J W Chamberlin, E Z Gordee

    Antimicrobial Agents and Chemotherapy
    |October 1, 1973
    PubMed
    Summary

    Monensin antibiotic biosynthesis by Streptomyces cinnamonensis involves acetate, propionate, and butyrate precursors. Methionine provides the o-methyl group, and acetate contributes the terminal hydroxymethyl group.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A Health Inequalities Impact Assessment of the surveillance of COVID-19 in asymptomatic patients attending dental settings in Scotland.

    Community dental health·2022
    Same author

    An examination of labor time-use on spring-calving dairy farms in Ireland.

    Journal of dairy science·2022
    Same author

    The Scattered Light Time-history Diagnostic suite at the National Ignition Facility.

    The Review of scientific instruments·2021
    Same author

    Evaluating the association of allergies with multiple sclerosis susceptibility risk and disease activity in a pediatric population.

    Journal of the neurological sciences·2017
    Same author

    Tabletop microsurgical training setup for the price of a pair of loupes.

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2017
    Same author

    The V-Y capsulotomy release for correcting capsular contracture.

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2015

    Area of Science:

    • Microbiology
    • Biochemistry
    • Metabolic pathways

    Background:

    • Monensin is a polyether ionophore antibiotic produced by Streptomyces cinnamonensis.
    • Understanding the biosynthesis of monensin is crucial for optimizing its production and exploring related compounds.

    Purpose of the Study:

    • To elucidate the biosynthetic pathway of monensin in Streptomyces cinnamonensis.
    • To identify the specific precursor molecules utilized in monensin synthesis.

    Main Methods:

    • Utilized carbon-14 (14C)-labeled precursors including glucose, acetate, propionate, butyrate, and methionine.
    • Analyzed the incorporation of labeled atoms into the monensin structure.

    Main Results:

    • Monensin is synthesized from five acetate units, seven propionate units, and one butyrate unit.
    • The o-methyl group of monensin originates from methionine.
    • The terminal hydroxymethyl group is derived from acetate.

    Conclusions:

    • The study clarifies the specific contributions of acetate, propionate, butyrate, and methionine to the monensin structure.
    • Provides a detailed understanding of the polyketide and methyl group origins in monensin biosynthesis.

    Related Experiment Videos