Isolation and identification of minor components of commercial kanamycin

A fraction enriched in the minor components of the kanamycin complex was prepared from a commercial sample. The enriched fraction was subjected to Amberlite CG-50 and Dowex 1-X2 column chromatography. Two minor components were isolated in addition to the known A, B, and C components. These congeners were identified as paromamine and 6-o-(3-amino-3-deoxy-alpha-d-glucopyranosyl)- deoxystreptamine. The identification was based on degradation and mass spectral studies.