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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Related Experiment Video

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Assaying Protein Kinase Activity with Radiolabeled ATP
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Simple assay for prekallikrein activators

A Bagdasarian, R C Talamo, R W Colman

    Immunological Communications
    |January 1, 1973
    PubMed
    Summary

    No abstract available in PubMed .

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