[The effect of acute fatigue on organ proteins and protein fractions]

Fatigue till exhaustion and renunciation was caused in rats through forced loaded swimming performance. Changes in watersoluble protein, in its fractions separated by means of disk electrophoresis, as well as in the fractions of lactic dehydrogenase and non-specific esterases were searched for in the different organs. Insofar water-soluble protein is concerned, a statistically reliable increase was found in the liver and heart chamber musculature, and reduction - in the skeletal muscles. Changes in the brain and kidney were not recorded. Large protein fraction ammassments were observed in all the organs which interfered with the interpretation of electrophoregrams. Nevertheless, clear-cut changes after exertion were not disclosed. The isoenzymes of lactic dehydrogenase did not reveal alterations in the organs investigated after physical effort. A loss of non-specific esterase fractions at electrophoretic range from 0.4 to 0.6 was established in the liver of tired animals only.