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Related Experiment Videos

Superoxide dismutase activity in leukocytes.

L R DeChatelet, C E McCall, L C McPhail

    The Journal of Clinical Investigation
    |April 1, 1974
    PubMed
    Summary
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    Superoxide dismutase enzyme activity was found in human neutrophils and rabbit macrophages. This enzyme protects phagocytic cells from superoxide generated during bacterial killing.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Immunology

    Background:

    • Phagocytic cells, such as neutrophils and macrophages, play a crucial role in the innate immune system.
    • The generation of superoxide anion is a key component of the microbicidal activity of these cells.
    • Understanding the cellular mechanisms that regulate superoxide levels is important for comprehending host defense.

    Purpose of the Study:

    • To identify and characterize superoxide dismutase activity in human neutrophils and rabbit alveolar macrophages.
    • To investigate the cellular localization and properties of this enzyme.
    • To assess the potential role of superoxide dismutase in protecting phagocytic cells during the inflammatory response.

    Main Methods:

    • Enzyme assays were performed using two distinct procedures to measure superoxide dismutase activity.

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  • Cellular fractions were analyzed to determine the subcellular localization of the enzyme.
  • The enzyme's sensitivity to inhibitors like cyanide and azide was tested.

    • Main Results:

    • Superoxide dismutase activity was successfully identified in both human neutrophils and rabbit alveolar macrophages.
    • The enzyme was found to be located in the cytosol of these phagocytic cells.
    • The identified superoxide dismutase was insensitive to inhibition by cyanide and azide.

    Conclusions:

    • The presence of superoxide dismutase in phagocytic cells supports the hypothesis that superoxide anion is involved in cellular bactericidal activity.
    • This enzyme likely functions as a protective mechanism, mitigating oxidative stress caused by superoxide generation during phagocytosis.
    • Further research into superoxide dismutase activity can elucidate its role in immune cell function and host defense against pathogens.