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Mesosome structure in Chromobacterium violaceum.

T E Rucinsky, E H Cota-Robles

    Journal of Bacteriology
    |May 1, 1974
    PubMed
    Summary
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    Chromobacterium violaceum, a gram-negative bacterium, exhibits frequent cytoplasmic membrane invaginations resembling mesosomes. These structures can significantly increase cell membrane surface area, offering new insights into bacterial cell biology.

    Area of Science:

    • Microbiology
    • Cell Biology
    • Bacterial Ultrastructure

    Background:

    • Gram-negative bacteria possess complex membrane structures.
    • Mesosomes are membrane invaginations typically observed in gram-positive bacteria.

    Purpose of the Study:

    • To investigate the presence and characteristics of mesosome-like structures in Chromobacterium violaceum.
    • To understand the association of these structures with the cytoplasmic membrane.

    Main Methods:

    • High-frequency electron microscopy of exponentially growing Chromobacterium violaceum cells.
    • Cellular treatments including washing with tris(hydroxymethyl)aminomethane buffer and plasmolysis.

    Main Results:

    • Observed frequent cytoplasmic membrane invaginations in Chromobacterium violaceum, resembling mesosomes.

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  • Identified up to four mesosomes per cell, potentially increasing membrane surface area by 30%.
  • Demonstrated distension of mesosome necks and cytoplasmic membranes upon buffer washing, and mesosome eversion during plasmolysis.

    • Conclusions:

    • Chromobacterium violaceum exhibits mesosome-like structures, challenging the typical classification of mesosomes in gram-positive bacteria.
    • These findings suggest a potential role for mesosomes in membrane dynamics and surface area regulation in gram-negative bacteria.